MersV1, Main, Exploration, bibRecord, 004956

A polymerase chain reaction assay adapted to plum pox potyvirus detection

Identifieur interne : 004956 ( Main/Exploration ); précédent : 004955; suivant : 004957

A polymerase chain reaction assay adapted to plum pox potyvirus detection

Auteurs : T. Wetzel [France] ; T. Candresse [France] ; M. Ravelonandro [France] ; J. Dunez [France]

Source :

Journal of Virological Methods [ 0166-0934 ] ; 1991.

RBID : ISTEX:BB7D398E9B016DABC9E669D31290DFD69A67F639

English descriptors

Teeft :
- Alui restriction site, Amar, Amar strain, Amplification, Annealing temperatures, Assay, Chemical denaturation, Clone, Clone pbppv1, Complete nucleotide sequence, Crude plant extracts, Detection limit, Dunez, Elisa test, Enzymatic amplification, Ethidium bromide staining, Field indexing trial, Fragment, Healthy peach extracts, Healthy sample, Hybridization, Hybridization detection, Lower amounts, Lower detection level, Methyl mercury hydroxide, Molecular hybridization, Molecular hybridization assay, Molecular weight standards, Nucleic acids, Nucleotide sequence, Pbppv1, Peach, Peach seedlings, Plant extracts, Plum, Polymerase, Polymerase chain reaction, Potyvirus, Primer, Probe pbppv1, Protein genes, Restriction analysis, Restriction endonucleases, Restriction profile, Restriction sites, Room temperature, Rsai, Silver staining, Small strips, Southern france, Sterile water, Target copies, Target sequence, Teycheney, Transcription mixture, Varveri, Viral, Viral particles, Virol, Wetzel.

Abstract

Abstract: A sensitive, polyvalent assay based on the polymerase chain reaction (PCR) was developed for plum pox potyvirus (PPV) detection. This technique was adapted for a single tube, the chemical denaturation and reverse transcription of the viral RNA followed by the PCR reaction yielding a 243-base-pair product. As few as 10 fg of purified viral RNA, corresponding to approximately 2000 viral particles, were detected in plant extracts. All PPV isolates tested were amplified, and the amplified fragments were analysed by restriction endonuclease digestion. An Rsal restriction site polymorphism in the amplified fragments allowed the discrimination of two groups of isolates. In a field indexing trial, the PCR assay proved to be more sensitive than molecular hybridization using 32P-labelled RNA probes for PPV detection.

Url:

https://api.istex.fr/ark:/67375/6H6-00P36L1Z-W/fulltext.pdf

DOI: 10.1016/0166-0934(91)90035-X

Affiliations:

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Le document en format XML

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<term>Assay</term>
<term>Chemical denaturation</term>
<term>Clone</term>
<term>Clone pbppv1</term>
<term>Complete nucleotide sequence</term>
<term>Crude plant extracts</term>
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<term>Dunez</term>
<term>Elisa test</term>
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<term>Field indexing trial</term>
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<term>Healthy sample</term>
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<term>Lower detection level</term>
<term>Methyl mercury hydroxide</term>
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<term>Molecular hybridization assay</term>
<term>Molecular weight standards</term>
<term>Nucleic acids</term>
<term>Nucleotide sequence</term>
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<term>Polymerase chain reaction</term>
<term>Potyvirus</term>
<term>Primer</term>
<term>Probe pbppv1</term>
<term>Protein genes</term>
<term>Restriction analysis</term>
<term>Restriction endonucleases</term>
<term>Restriction profile</term>
<term>Restriction sites</term>
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<term>Rsai</term>
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<term>Southern france</term>
<term>Sterile water</term>
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<term>Target sequence</term>
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<term>Transcription mixture</term>
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<front><div type="abstract" xml:lang="en">Abstract: A sensitive, polyvalent assay based on the polymerase chain reaction (PCR) was developed for plum pox potyvirus (PPV) detection. This technique was adapted for a single tube, the chemical denaturation and reverse transcription of the viral RNA followed by the PCR reaction yielding a 243-base-pair product. As few as 10 fg of purified viral RNA, corresponding to approximately 2000 viral particles, were detected in plant extracts. All PPV isolates tested were amplified, and the amplified fragments were analysed by restriction endonuclease digestion. An Rsal restriction site polymorphism in the amplified fragments allowed the discrimination of two groups of isolates. In a field indexing trial, the PCR assay proved to be more sensitive than molecular hybridization using 32P-labelled RNA probes for PPV detection.</div>
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A polymerase chain reaction assay adapted to plum pox potyvirus detection

A polymerase chain reaction assay adapted to plum pox potyvirus detection

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri